| 3 | 0 | 49 |
| 下载次数 | 被引频次 | 阅读次数 |
目的:通过建立动物模型,探讨放射性治疗对SD大鼠唾液中变形链球菌数量的影响。方法:选自20只雌性SD大鼠,随机分成试验组和对照组,每组各10只。经接种变形链球菌后,试验组予以15 Gy/d放射剂量对双侧腮腺进行照射,连续照射30 d。于放射始、30、60 d以及90 d时,观察大鼠唾液中变形链球菌的数量,并于放射90 d时,统计患龋率及根据Keyes评分法评定大鼠患龋状况。结果:随着观察时间的延长,两组大鼠唾液中变形链球菌的数量均不断增加,但实验组各时间点变形链球菌增加数量明显高于对照;放射90 d时,大鼠唾液变形链球菌的数量可达[(8.60±0.21)×107]mL,而对照组仅为1(1.91±0.12)×107]mL,两组间差异有统计学意义(P<0.05)。实验组的惠龋率及Keyes评分法均明显高于对照组,两组间差异有统计学意义(P<0.05)。结论:放疗可促进SD大鼠唾液中变形链球菌的繁殖而增加其患龋的风险。
Abstract：Objective:To explore the effect of radiotherapy on level of Streptococcus mutans in saliva of established Sprague-Dawley(SD) rat models.Methods:Twenty female SD rats were randomized into the experimental group and the control group(n=10 each).The rats were inoculated with Streptococcus mutans. Thereafter,the experimental group was exposed to 15Gy/d radiation of bilateral parotid glands for 30 days.The counts of Streptococcus mutans in saliva of SD rats were record on days 1,30,60 and 90 of treatment. Prevalence and severity of dental caries in SD rats were evaluated according to Keyes caries scores.Results:The counts of Streptococcus mutans in both groups increased over time,but were higher in the experimental group compared with the control group at each time point.On day 90 post-radiation,the count of Streptococcus mutans was(8.60±0.21)×107 ml in the experimental group vs(1.91±0.12)×107 ml in the control group,with statistical significance between the two(P<0.05).The prevalence and Keyes scores of caries were also higher in the experimental group than those in the control group(P < 0.05 ).Conclusion:Radiotherapy may stimulate growth of Streptococcus mutans in saliva and increase risk of caries in SD rats.
[1]Hong CH,Napenas JJ,Hodgson BD,et al.A systematic review of dental disease in patients undergoing cancer therapy[J].Support Care Cancer,2010,18(8 ):1007- 1021.
[2]Chambers MS,Tomsett KL,ArtopoulouⅡ,et al.Salivary flow rates measured during radiation therapy in head and neck cancer patients:a pilot study assessing salivary sediment formation[J].J Prosthet Dent,2008,100(2): 142-146.
[3]Omori Y,Asari T,Maruyama K,et al.Effects of pilocarpine hydrochloride and cevimeline on submandibnlar/sublingual salivation in rat xerostomia model produced by X-rayirradiation[J].Arzneimittelforschung, 2003,53(5):342-350.
[4]Takagi Y,Katayama I,Tashiro S,et al.Parotid irrigation and cevimeline gargle for treatment of xerostomia in Sjogren' s syndrome[J].J Rheumatol,2008,35(11 ): 2289-2291.
[5]Keyes PH.Dental caries in the molar teeth of rats.Ⅰ. Distribution of lesions induced by high-carbohydrate low-fat diets[J].JDent Res,1958,37(6):1077-1087.
[6]Al-Nawas B,Gr tz KA.Prospective study of the long term change of the oral flora after radiation therapy[J].Support Care Cancer,2006,14(3 ):291-2966.
[7]郑宝敏,孙艳.放射性龋齿相关因素研究[J].国外医学临床放射学分册,2006,29(4):280-284.
[8]Meng L,Liu J,Peng B,et al.The persistence of Streptococcus mutans in nasopharyngeal carcinoma patients after radiotherapy[J].Caries Res,2005,39(6):484-489.
[9]Vuotila T,Ylikonntiola L,Sorsa T,et al.The relationship between MMPs and Phin whole saliva of radiated head and neck cancer patients[J].J Oral Pathol Med,2002,31(6 ): 329-338.
[10]Hu JY,Li YQ,Smales R J,et al.Restoration of teeth with more-viscous glass ionomer cements following radiation-induced caries[J].Int Dent J,2005,52(6): 445 -448.
[11]张雪,李玉晶,王松灵,等.放射线对牙体硬组织及其抗酸溶解性影响的研究[J].中华口腔医学杂志, 2004,39(6):463-466.
[12]黄征难,陈发明.放射性龋的研究进展[J].中华放射医学与防护杂志,2003,23(6)480-481.
基本信息:
DOI：
中图分类号:R739.63
引用信息:
[1]刘江峰,陈之锋,高平等.放射性治疗对SD大鼠唾液中变形链球菌数量的影响[J].广州医学院学报,2011,39(04):35-37.
基金信息:
广东省自然科学基金(81510140002000002);; 广州市荔湾区科技计划项目(20081210079)