| 6 | 4 | 137 |
| 下载次数 | 被引频次 | 阅读次数 |
目的:探讨低聚糖阿魏酸酯(CFOs)在由链脲佐菌素诱导的1型糖尿病怀孕大鼠体内的降血糖和抗氧化作用。方法:取40只大鼠做实验,共分五组(每组8只):(1)未交配过的糖尿病鼠为对照组;(2)交配过的非糖尿病鼠为对照组;(3)交配过的糖尿病鼠为对照组;(4)未交配过的糖尿病鼠为治疗组;(5)交配过的糖尿病鼠为治疗组。治疗组在大鼠交配前3 d开始,连续15 d给大鼠喂饲玉米低聚糖阿魏酸酯植物提取物(25 mg/kg)。对照组动物则只接受溶媒处理(2.5 mL/kg)。在交配后的第14天,处死大鼠,从下腔静脉采集母体血液,做ELISA检测。交配过的大鼠应解剖其子宫以搜寻任何可能存在的胎儿。每只大鼠的肝脏都被迅速切除,用Jamall和Smith的检测方法来确定组织样本中脂质过氧化物的水平。结果:不考虑怀孕因素,低聚糖阿魏酸酯的使用可降低血糖值(P<0.05)。使用低聚糖阿魏酸酯治疗未孕和怀孕大鼠后,两者血清胰岛素水平均上升(P<0.05)。使用低聚糖阿魏酸酯并未对血浆内的硫代巴比妥酸反应物水平产生显著影响(P>0.05)。实验动物被诱导患糖尿病后,再用低聚糖阿魏酸酯进行治疗,其谷胱甘肽水平的平均值有所下降(P<0.05)。糖尿病孕鼠治疗组和孕鼠对照组肝脏内的硫代巴比妥酸反应物水平和谷胱甘肽水平,差异有统计学意义(P<0.05)。结论:低聚糖阿魏酸酯可降低怀孕及未怀孕糖尿病大鼠体内的血糖浓度、提高血清胰岛素浓度;与肝脏有关的脂质过氧化反应上升和谷胱甘肽的下降发生逆转。
Abstract：Objective:To investigate the hypoglycaemic and antioxidant effects of the corn feruloyl oligosaccharides(CFOs)in pregnant rat models with streptozotocin-induced type 1 diabetes.Methods:Five groups(n= 8,each)were formed:(1)diabetic non-mated control group;(2)non-diabetic mated control group;(3)diabetic mated control group;(4)diabetic non-mated treatment group and(5)diabetic mated treatment group. The CFOs plant extract(25 mg/ kg)was administered orally for 15 continuous days in treatment groups,beginning 3 days before the rats mating. And the control groups only accepted menstruum treatment(2.5mL/ kg). In the 14 th day after mating,all rats were executed and the maternal venous blood was collected from inferior vena cava for ELISA detection. Mated rats should be dissected to search any possible fetuses in theiruterus. The liver of each rat was quickly removed to determine the levels of lipid peroxide in tissue samples by detection method of Jamall and Smith.Results:Without the consideration of pregnant factors,the use of feruloyl oligosaccharides could decrease the value of blood glucose(P<0.05). After using feruloyl oligosaccharides to treat non-pregnant and pregnant rats,the levels of both serum insulin increased( P< 0. 05). And the use of feruloyl oligosaccharides had no significant influence on the levels of thiobarbituric acid-reactive substances of the serum(P>0.05). After the experimental animals with induced diabetes treated by feruloyl oligosaccharides,their mean level of glutathione decreased(P<0.05). There were statistical significance of the levels of thiobarbituric acid-reactive substances and the levels of glutathione between the rat livers of diabetic pregnant treatment group and pregnant control group(P<0.05).Conclusion:Feruloyl oligosaccharides could decrease the concentration of blood glucose and increase the concentration of serum insulin in pregnant and non-pregnant diabetic rats. And the rise of lipid peroxidation and the decline of glutathione related with liver reversed.
[1]Halliwell B.Biochemistry of oxidative stress[J].Biochem Soc Trans,2007,35(5):1147-1150.
[2]Dennery PA.Effects of oxidative stress on embryonic development.Birth Defects Research Part C[J].Embryo Today Reviews,2007,81(3):155-162.
[3]Franz MJ,Bantle JP,Beebe CA,et al.American Diabetes Association.Nutrition principles and recommendations in diabetes[J].Diabetes Care,2004,27:(Suppl)S36-S46.
[4]赵东平,杨文钰,陈兴福.阿魏酸的研究进展[J].时珍国医国药,2008,19(8):1839-1841.
[5]Quan DQ,Cui GH,Ruan JX.Inhibitory effects of sodium ferulate on proliferation of rabbit aortic smooth muscle cells induced by oxidized LDL[J].J Chin Pharm Sci,2001,10:208-211.
[6]Kurtel H,Granger DN,Tso P,Grisham MB.Vulnerability of intestinal interstitial fluid oxidant stress[J].Am J Physiol,1992,263(4):573-578.
[7]Jamall IS,Smith JC.Effects of cadmium on glutathione peroxidase,superoxide dismutase,and lipid peroxidation in the rat heart:a possible mechanism of cadmium cardiotoxicity[J].Toxicol Applied Pharmacol,1985,80(1):33-42.
[8]Jonasson JM,Brismar K,Spare NP,et al.Fertility in women with type 1 diabetes:a population-based cohort study in Sweden[J].Diabetes Care,2007,30(9):2271-2276.
[9]Murthy VK,Shipp JC.Regulation of hepatic triglyceride synthesis in diabetic rats[J].J Clin Investigat,1981,67(4):923-930.
[10]Hadjadj S,Duly-Bouhanick B,Bekherraz A,et al.Serum triglycerides are a predictive factor for the development and the progression of renal and retinal complications in patients with type 1 diabetes[J].Diabetes Metabolism,2004,30(1):43-51.
[11]Aslan M,Orhan D,Orhan N,et al.In vivo antidiabetic and antioxidant potential of Helichrysum plicatum.plicatum capitulums in streptozotocin induced-diabetic rats[J].J Ethnopharmacol,2007,109(1):54-59.
[12]Musabayane CT,Munjeri O,Mdege ND.Effects of Helichrysum ceres extracts on renal function and blood pressure in the rat[J].Renal Failure,2003,25(1):5-14.
基本信息:
中图分类号:R714.256
引用信息:
[1]秦庆新,肖正华,谢晓斌.低聚糖阿魏酸酯改善妊娠期糖尿病代谢控制及其肝氧化生物标记物的研究[J].广州医科大学学报,2014,42(04):30-34.
2014-08-15
2014-08-15