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目的:探讨凋亡抑制蛋白Survivin对膀胱癌T24细胞增殖、迁移和凋亡的影响。方法:克隆人凋亡抑制蛋白Survivin基因,构建慢病毒表达载体后感染膀胱癌细胞株T24。实验分为对照组、实验组(Survivin-LV)和阴性对照组(FU-RNAi-NC-LV)。Western印迹鉴定Survivin蛋白表达;MTT法、细胞划痕实验和流式细胞术检测下调Survivin的表达对膀胱癌T24细胞的增殖、迁移和凋亡的影响。结果:成功构建Survivin慢病毒表达载体,Western印迹显示实验组较阴性对照组细胞Surivin蛋白表达减少;实验组慢病毒感染细胞72 h后对细胞增殖的抑制率是明显高于其他组(P<0.01);细胞迁移实验发现,与对照组和阴性对照组比较,实验组的细胞迁移明显被抑制(P<0.01);流式细胞仪检测结果显示实验组的细胞凋亡率[(18.3±1.21)%]显著高于对照组[(2.3±0.69)%]和阴性对照组[(3.1±0.82)%](P<0.01)。结论:Survivin下调可使膀胱癌T24细胞增殖和迁移明显抑制,细胞凋亡增加。
Abstract：Objective:To study the effects of Survivin lower-expression in the process of proliferation and apoptosis in human bladder tumor cells(T24).Methods:The lentiviral vector of Survivin-LV was constructed and transfected into Human bladder cancer cells(T24).Western blot identified the express of Survivin protein.MTT assay,cell migratory and flow cytometry verified the the proliferation,migration and apoptosis while reducing expression the Survivin protein in in human bladder cancer cells.Result:Western blot showed that the expression of Surivin protein in the experimental group was lower compare with the negative control group.Both proliferation and migration of cells was significantly inhibited in experimental group with the control group and the negative control group(P<0.01).The results of flow cytometry showed that the apoptosis rate of the experimental group[(18.3±1.21) %]was significantly higher compared with the control group[(2.3±0.69) %] and the negative control group[(3.1±0.82) % ](P<0.01).Conclusion:Down-regulation of Survivin significantly inhibited both of the proliferation and migration,and promoted apoptosis in bladder cancer T24 cells.
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基本信息:
中图分类号:R737.14
引用信息:
[1]谢顺明,王文晓,顾雅娟,等.Survivin下调对膀胱癌细胞的增殖、迁移和凋亡的影响[J].广州医科大学学报,2018,46(03):20-24.
2018-06-15
2018-06-15