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目的：研究骨髓间充质干细胞(MSC)与分化抗原簇蛋白133(CD133)+肾脏细胞治疗急性肾损伤的机制。方法：选取6～8周龄雄性C57 BL/6的48只小鼠作为实验对象，根据不同的治疗方案将48只小鼠分为正常对照组、缺血再灌注损伤(I/R)组、I/R+MSCs组及I/R+CD133+组4组，每组12只。比较各组小鼠术后不同时间点血清尿素氮(BUN)与肌酐(Cr)水平、ATN评分、肿瘤坏死因子?α(TNF?α)、白细胞介素?10(IL?10)、肝细胞生长因子(HGF)及骨形态发生蛋白?7(BMP?7)水平。结果：I/R+MSCs组、I/R+CD133+组术后BUN、Cr水平、ATN评分、TNF?α水平均显著高于正常对照组；术后4、7 d的BUN、Cr水平、ATN评分、TNF?α水平均低于I/R组，其中I/R+MSCs组以术后8 d最为显著(P<0.05);I/R+CD133+组以术后4 d最为显著(P<0.05)。I/R+MSCs组、I/R+CD133+组术后IL?10、HGF、BMP?7水平均低于正常对照组，高于I/R组(P<0.05)。与I/R+MSCs组比较，I/R+CD133+组术后BUN、Cr水平，ATN评分及TNF?α水平较低，IL?10、HGF、BMP?7水平较高。结论：骨髓MSCs、CD133+肾脏细胞均可通过内分泌方式调控细胞因子，促进由I/R诱导的AKI恢复，但CD_+133+肾脏细胞对AKI的修复作用更为显著。
Abstract：Objective:To determine the mechanism of bone marrow mesenchymal stem cells(MSCs)and cluster of differentiation 133(CD133)~+kidney cells in the treatment of acute kidney injury.Methods:Forty-eight male C57 BL/6 mice aged 6-8 weeks old were included as the subjects in the study.According to different treatment methods,all mice were divided into the normal control group,ischemia-reperfusion injury(I/R)group,I/R+MSCs group,and I/R+CD133~+group(n=12 each).The serum urea nitrogen(BUN)and creatinine(Cr)levels,ATN score,tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),hepatocyte growth factor(HGF)and bone morphogenetic protein-7(BMP-7)levels of mice in each group were compared at different time points after the operation.Results:The postoperative BUN and Cr levels,ATN score,and TNF-αlevel in the I/R+MSCs group and I/R+CD133~+group were significantly higher than those in the normal control group.The BUN and Cr levels,ATN score,and TNF-α level in the I/R+MSCs group and I/R+CD133~+group were lower than those in the I/R group at 4d and 7d after operation;these were more significant with the I/R+MSCs group at 8d after operation(allP<0.05),and with the I/R+CD133~+group at 4d after operation(allP<0.05).The postoperative IL-10,HGF and BMP-7 levels in the I/R+MSCs group and the I/R+CD133~+group were lower than those in the normal control group and higher than those in the I/R group(allP<0.05).Compared with the I/R+MSCs group,the postoperative BUN and Cr levels,ATN score and TNF-α level were lower in the I/R+CD133~+group,whereas the IL-10,HGF,and BMP-7 levels were higher.Conclusion:Both bone marrow MSCs and CD133~+kidney cells may regulate cytokines in an endocrine manner and promote the recovery of I/R induced AKI.Comparatively,the repair effect of CD133~+kidney cells on AKI is more significant.
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基本信息:
中图分类号:R692
引用信息:
[1]黄远航,范立明,黄盈,等.骨髓间充质干细胞与CD133~+肾脏细胞治疗急性肾损伤的机制研究[J].广州医科大学学报,2022,50(05):6-11.
基金信息:
广州市科技计划项目（202102080599）